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Modelling random antibody adsorption and immunoassay activity

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  • One of the primary considerations in immunoassay design is optimizing the concentration of capture antibody in order to achieve maximal antigen binding and, subsequently, improved sensitivity and limit of detection. Many immunoassay technologies involve immobilization of the antibody to solid surfaces. Antibodies are large molecules in which the position and accessibility of the antigen-binding site depend on their orientation and packing density.
        In this paper we propose a simple mathematical model, based on the theory known as random sequential adsorption (RSA), in order to calculate how the concentration of correctly oriented antibodies (active site exposed for subsequent reactions) evolves during the deposition process. It has been suggested by experimental studies that high concentrations will decrease assay performance, due to molecule denaturation and obstruction of active binding sites. However, crowding of antibodies can also have the opposite effect by favouring upright orientations. A specific aim of our model is to predict which of these competing effects prevails under different experimental conditions and study the existence of an optimal coverage, which yields the maximum expected concentration of active particles (and hence the highest signal).
    Mathematics Subject Classification: Primary: 97M60, 92C50, 92C45; Secondary: 60K40, 92C40.

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  • [1]

    D. A. Edwards, Steric hindrance effects on surface reactions: Applications to BIAcore, J. Math. Biol., 55 (2007), 517-539.doi: 10.1007/s00285-007-0093-7.

    [2]

    J. W. Evans, Random and cooperative sequential adsorption, Rev. Mod. Phys., 65 (1993), 1281.doi: 10.1103/RevModPhys.65.1281.

    [3]

    V. Gubala, C. Crean, R. Nooney, S. Hearty, B. McDonnell, K. Heydon, R. O'Kennedy, B. D. MacCraith and D. E. Williams, Kinetics of immunoassays with particles as labels: Effect of antibody coupling using dendrimers as linkers, Analyst, 136 (2011), 2533-2541.doi: 10.1039/c1an15017k.

    [4]

    M. K. Hassan, J. Schmidt, B. Blasius and J. Kurths, Jamming coverage in competitive random sequential adsorption of a binary mixture, Phys. Rev. E, 65 (2002), 045103.doi: 10.1103/PhysRevE.65.045103.

    [5]

    P. L. Krapivsky, Kinetics of random sequential parking on a line, J. Stat. Phys., 69 (1992), 135-150.doi: 10.1007/BF01053786.

    [6]

    A. Rényi, On a one-dimensional problem concerning random space-filling (In Hungarian), Publ. Math. Inst. Hung. Acad. Sci, 3 (1958), 109-127.

    [7]

    J. C. Roach, V. Thorsson and A. F. Siegel, Parking strategies for genome sequencing, Genome Research, 10 (2000), 1020-1030.doi: 10.1101/gr.10.7.1020.

    [8]

    B. Saha. T. H. Evers and M. W. J. Prins, How antibody surface coverage on nanoparticles determines the activity and kinetics of antigen capturing for biosensing, Anal. Chem., 86 (2014), 8158-8166.

    [9]

    W. Schramm and S. Paek, Antibody-antigen complex formation with immobilized immunoglobulins, Anal. Biochem., 205 (1992), 47-56.doi: 10.1016/0003-2697(92)90577-T.

    [10]

    J. Talbot, G. Tarjus, P. R. Van Tassel and P. Viot, From car parking to protein adsorption: An overview of sequential adsorption processes, Colloids Surf. A, 165 (2000), 287-324.doi: 10.1016/S0927-7757(99)00409-4.

    [11]

    M. L. M. Vareiro, J. Liu, W. Knoll, K. Zak, D. Williams and A. T. A. Jenkins, Surface plasmon fluorescence measurements of human chorionic gonadotrophin: role of antibody orientation in obtaining enhanced sensitivity and limit of detection, Anal. Chem., 77 (2005), 2426-2431.doi: 10.1021/ac0482460.

    [12]

    D. Wild (editor), The Immunoassay Handbook, $3^{rd}$ edition, Elsevier, 2005.

    [13]

    M. E. Wiseman and C. W. Frank, Antibody adsorption and orientation on hydrophobic surfaces, Langmuir, 28 (2012), 1765-1774.doi: 10.1021/la203095p.

    [14]

    H. Xu, J. R. Lu and D. E. Williams, Effect of surface packing density of interfacially adsorbed monoclonal antibody on the binding of hormonal antigen human chorionic gonadotrophin, J. Phys. Chem. B, 110 (2006), 1907-1914.doi: 10.1021/jp0538161.

    [15]

    H. Xu, X. Zhao, C. Grant, J. R. Lu, D. E. Williams and J. Penfold, Orientation of a monoclonal antibody adsorbed at the solid/solution interface: A combined study using atomic force microscopy and neutron reflectivity, Langmuir, 22 (2006), 6313-6320.doi: 10.1021/la0532454.

    [16]

    X. Zhao, F. Pan, B. Cowsill, J. R. Lu, L. Garcia-Gancedo, A. J. Flewitt, G. M. Ashley and J. Luo, Interfacial immobilization of monoclonal antibody and detection of human prostate-specific antigen, Langmuir, 27 (2011), 7654-7662.doi: 10.1021/la201245q.

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